Photosynthetic assimilation and translocation of 3H- and 14C-organic compounds after 3HHO and 14CO2 were simultaneously offered to a primary leaf of soybean

Abstract

After assimilation of both 3HHO and 14CO2 by soybean (Glycine max L.) in the light, the percentage of nonexchangeable 3H was similar to the percentage of 14C distributed in each of the identified sugars, amino acids, and organic acids with the exceptions of glycine–serine and glutamic acids. Glycine–serine contained proportionally more 14C than expected on the basis of percentage of 3H; and glutamic acid contained proportionally more 3H than expected on the basis of 14C. Both 3H- and 14C-organic compounds were translocated in the same proportions to various parts of the plant and in both cases the main compound translocated was apparently sucrose. The metabolism of dark-assimilated 3H was different from dark-assimilated 14C, but in both cases it did not contribute significantly to the total metabolism of the plant.