Improving drought tolerance of Opuntia ficus-indica under field using subsurface water retention technology: changes in physiological and biochemical parameters

Abstract

The prickly pear ( Opuntia ficus-indica) is an essential economic and ecological medicinal plant in arid and semi-arid areas, especially in Morocco, where water scarcity affects its survival and growth. Evidence suggests that subsurface water retention technology (SWRT) may enhance crops’ drought resistance. A field experiment was conducted to explore SWRT application effects on cactus cladodes’ growth and physiological and biochemical performance under drought conditions. Two water regimes were applied (rainfed conditions; irrigated with 4 L of water twice a week) with two treatments (without SWRT; with SWRT). The results showed that cactus cladodes’ growth and physiological and biochemical parameters cultivated for 8 months were negatively affected by drought. Drought-exposed cactus cladodes under SWRT application showed an increase in surface area and cladode stomatal densities by 65% and 29%, respectively, compared with no SWRT. This technology reduced drought-induced oxidative stress by mitigating malondialdehyde and hydrogen peroxide excess by 22% and 17%, respectively. Moreover, lower levels of enzymatic and nonenzymatic antioxidant activities were concluded, and soil organic matter and assimilable phosphorus contents were enhanced. In conclusion, our findings highlighted SWRT’s positive impacts on the tested parameters, thus presenting it as a promising technology for cactus growth and development improvement under water deficiency.