Functional analysis of three AHL autoinducer synthase genes in Mesorhizobium loti reveals the important role of quorum sensing in symbiotic nodulation

Author:

Yang Menghua123,Sun Kejing123,Zhou Lei123,Yang Ruifu123,Zhong Zengtao123,Zhu Jun123

Affiliation:

1. Department of Microbiology, MOA Key Laboratory of Microbiological Engineering of Agricultural Environment, Nanjing Agricultural University, Nanjing, China.

2. Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, China.

3. The State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, China.

Abstract

One of the most important signal transduction pathways in bacteria, quorum sensing, is involved in many regulatory circuits in rhizobia, especially in the control of communication between rhizobia and their plant hosts. In this study, we identified 3 autoinducer synthase genes — mrlI1, mrlI2, and mrlI3 — in Mesorhizobium loti NZP 2213. We found that MrlI1 and MrlI2 could synthesize distinct N-acyl homoserine lactone (AHL) autoinducers in rich medium cultures, and the expression of mrlI1 was shown to be growth-phase-dependent. MrlI3 did not produce any detectable AHL molecules under the culture conditions tested. To investigate whether these AHL synthases affect nodulation, we examined the nodulation of AHL-deficient mutants on their native plant host Lotus corniculatus and found that the efficiency of nodulation of bacteria with mutations of any of these 3 synthase genes was reduced, suggesting that quorum sensing systems in M. loti may play an important role in successful establishment of rhizobium–legume symbiosis.

Publisher

Canadian Science Publishing

Subject

Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology

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