Metabolism of Estrogens and Their Sulfates by Mouse Kidney Tissue in Vitro

Abstract

Whole kidney tissue from mouse was incubated with labelled estrone 3-sulfate (E13S), 17β-estradiol 3-sulfate (17βE23S), estrone (E1), and 17β-estradiol (17βE2). Considerable reduction of E13S and E1 occurred. E13S gave rise primarily to 17α-estradiol 3-sulfate (17αE23S) together with lesser amounts of 17βE23S. By incubating [3H-35S]E13S with the tissue it was confirmed that formation of the diol sulfates was direct, without accompanying hydrolysis and reconjugation. Conversion of E1 was mainly to 17βE2 with, on the average, lesser amounts of 17αE2. A small degree of direct conversion of 17βE23S to E13S was found. 17β-Estradiol was converted to a limited extent to E1 and to much smaller amounts of 17αE2.