Wheat embryo ribonucleates. XII. Formal characterization of terminal and penultimate nucleoside residues at the 5′-ends of 'capped' RNA from imbibing wheat embryos

Abstract

(1) N2,N2,7-Trimethylguanosine, not previously detected as a component of plant RNA, is shown to be present in the RNA which is isotopically labelled when dry wheat embryos imbibe water in a medium that contains [methyl-3H]methionine.(2) N2,N2,7-Trimethylguanosine and 7-methylguanosine are released as part of 'capped' oligonucleotides when the isotopically labelled RNA from imbibing wheat embryos is subjected to hydrolysis by RNase T2.(3) By way of contrast with the 'capped' RNA of animal cells, 5′-terminal 'cap' structures (m7Gppp- and m32,2,7Gppp-) in the 'capped' RNA from the higher plant organism are not bonded to penultimate O2′-methylnucleoside constituents.(4) In an allied study, it has been found that recovery of poly(A)-rich RNA from dry wheat embryos depends on the inclusion of sodium dodecyl sulphate (SDS) in phosphate-buffered (pH 6.8) phenolic emulsions. By way of contrast, recovery of poly(A)-rich RNA from dry wheat embryos does not depend on the inclusion of SDS in Tris(hydroxymethyl)aminomethane buffered (pH 9.0) phenolic emulsions.