3H-2-glucose as tracer in turnover studies

Abstract

In rats the reported difference between the slopes of the specific activities of plasma glucose following i.v. injection of either 3H-2-glucose or 14C-1-glucose is abolished by evisceration and nephrectomy. This proves that the difference observed in normal rats is due to the reincorporation of 14C atoms into newly released glucose and not to a non-metabolic tritium–proton exchange in plasma glucose.