Photochemical interaction of dictamnine, a furoquinoline alkaloid, with fungal DNA in vitro and in vivo

Abstract

The furoquinoline alkaloid dictamnine has been shown to provoke lethal damage to filamentous fungi in near ultraviolet light. The phototoxicity was more pronounced against Mucor hiemalis and Mucor ramannianus than against Fusarium graminearum and Penicillium italicum. In vitro, labeled dictamnine was shown to form covalent monoadducts with purified DNA from M. hiemalis in the presence of long-wave ultraviolet light. Addition of [3H]dictamnine to cultures of the same organism showed photobinding with the fungal DNA in vivo. These results support the suggestion made earlier, on the basis of in vitro experiments with calf thymus DNA, that DNA represents a major cellular target in vivo for the phototoxicity of the alkaloid.