Author:
Bhuyan B. K.,Simpson F. J.
Abstract
The D-xylulokinase of Aerobacler aerogenes is an inducible enzyme specific for its substrate. The optimum pH for activity lies between 7.5 and 8.7. At 30 °C the enzyme is most stable between pH 6.0 and 9.5 and is inactivated at pH 4.6 and 11.5 within 15 minutes. At 61 °C, pH 7.8, 41% of the enzyme is inactivated in 15 minutes and at 66 °C completely inactivated. Magnesium ions, although required for activity, labilize the enzyme, while D-xylulose, adenosine triphosphate, EDTA, and glycerol protect the enzyme. The enzyme is sensitive to heavy metals and sulphhydryl reagents. ITP and GTP are about 25% and UTP about 14% as effective as ATP as phosphate donors.
Publisher
Canadian Science Publishing
Subject
Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology
Cited by
11 articles.
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