Author:
Li Qin,Briggs M Martha,Folkens Doris,Hill Ceredwyn E
Abstract
Bile acid independent flow composes up to 40% of hepatic bile secretory capacity. Apical (canalicular) efflux of non-bile-acid organic anions provides the major osmotic driving force for bile acid independent flow. Organic anion accumulation in the hepatocyte is accompanied by increases in both K+conductance in isolated hepatocytes and passive K+flux in the perfused rat liver, which are indicative of K+channel activation. We used two models of disrupted canalicular anion transport to test whether organic anion stimulated K+efflux occurs independently of anion excretion. In both wild type (wt) and mrp2 mutant (transport minus, tr) rat liver, bromosulfophthalein (BSP; 0.5mM) caused a reversible increase in K+flux that (i) was outwardly directed with low external K+and (ii) depended upon the electrochemical potential for K+. K+efflux from wt livers of both sexes was about 1.5 times larger than that from trlivers. Further, K+release from female rat livers was about three times higher than that from male livers, independent of phenotype. Two transcripts of the rat hepatocyte K+channel (Kir4.2) were expressed in hepatocytes of all rats. The results demonstrate that BSP stimulates basolateral (sinusoidal) K+channels independently of its canalicular excretion, revealing an early event in BAIF and suggesting that Kir4.2 may mediate BSP-sensitive K+flux.Key words: liver, GY, gender-specific, male, female, transport minus, Kir4.2.
Publisher
Canadian Science Publishing
Subject
Physiology (medical),Pharmacology,General Medicine,Physiology
Cited by
3 articles.
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