Purification and some properties of aspartate aminotransferase of Amycolatopsis methanolica

Abstract

Aspartate aminotransferase (EC 2.6.1.1) from Amycolatopsis methanolica was purified and characterized. It is a dimeric protein with an overall molecular weight of approximately 90 000 with maximal activity at 70 °C and at pH 8.5. The enzyme was specific for L-aspartate as amino donor and 2-ketoglutarate as amino acceptor: the Km values for L-aspartate and 2-ketoglutarate were 1.27 and 0.38 mM, respectively.Key words: Amycolatopsis methanolica, aspartate aminotransferase, L-aspartate, 2-ketoglutarate.