Les systèmes membranaires de Scopulariopsis brevicaulis. I. Isolement et caractérisation

Abstract

Membranes isolated (except mitochondrial membranes) from mycelium of Scopulariopsis brevicaulis were separated by continuous density gradient centrifugation using Percoll at a concentration of 10%. This method was compared with discontinuous sucrose density gradient centrifugation. Isolated membrane systems were characterized by enzymatic markers (NADPH and NADH cytochrome c reductases, ATPase, AMPase, IDPase) and by cytochemical reactions using electron microscopy (Thiéry test, phosphotungstic acid – chromic acid). The best separation of membranes was obtained with continuous density gradient centrifugation using 10% Percoll.