Dose-responses of Bacillus cereus RS87 for growth enhancement in various Thai rice cultivars

Abstract

To achieve the goal of reducing the levels of chemical fertilizers applied in rice production, there is a need to develop microorganisms with the capacity to enhance plant growth. Previous studies have demonstrated that Bacillus cereus RS87 promotes growth of various plants in greenhouse and field trials. The objectives of this study were to (i) evaluate the efficacy and determine the optimum concentration of B. cereus RS87 to enhance growth of various Thai rice cultivars, (ii) measure the chlorophyll content in leaves affected by strain RS87, and (iii) investigate the capacity of strain RS87 to solubilize phosphate and produce siderophores. Three concentrations of strain RS87 (log 6.0, log 7.0, and log 8.0 CFU/mL) were applied to each rice cultivar. Superior responses (i.e., enhanced development of roots and shoots of all rice cultivars) were observed using RS87 at log 8.0 CFU/mL compared with lower bacterial concentrations and the water-treated control treatment. In addition, log 8.0 CFU/mL of RS87 provided the greatest root length and plant height of all rice cultivars 45 days after planting in the greenhouse. Rice leaves treated with log 8.0 CFU/mL of RS87 yielded the highest total chlorophyll, specifically chlorophyll a and chlorophyll b, compared with the control. Strain RS87 also solubilized phosphate and produced siderophores. The results of these studies demonstrate that log 8.0 CFU/mL is the optimum concentration of strain RS87 for growth promotion of various Thai rice cultivars.