Biocapteurs ampérométriques à cholinestérases pour la détermination des pesticides organophosphorés

Abstract

The purpose of this study is a comparative presentation of the different types of the amperometric biosensors based on cholinesterases for the determination of organophosphorous pesticides using the bibliographical information of the last 20 years. The study contains the presentation of the structure and properties of the cholinesterases, the main reactions implied in the functioning of the amperometric biosensors, their applications and factors influencing the detection or (and) the inhibition process. The detection limit of the mono- or bi-enzymatic amperometric biosensors are relatively higher than those corresponding with the immunobiosensors or with gas and liquid chromatography, which are still considered as the reference methods. As shown, for many other amperometric biosensors, the Michaelis–Menten's kinetic treatment used for reactions catalyzed by free enzymes can be extended to describe the response of amperometric biosensors based on immobilized cholinesterases. The positive compromise between advantages and drawbacks, as well as the "soft" experimental conditions, point to the amperometric monoenzymatic bioelectrode, as an attractive analytical tool for the detection of organophosphorous pesticides.Key words: amperometric biosensor, acetylcholinesterase, organophosphorous pesticides, kinetic, inhibition.