Cellular differentiation and peroxidase isozymes in cell culture of peanut cotyledons

Abstract

A suspension culture obtained from peanut (Arachis hypogaea L.) cotyledons was passed through three standard sieves of 0.15, 0.5, and 2.0 mm and the subsequent four fractions were studied for morphological differences and molecular heterogeneity in peroxidase isozymes. Quantitative and qualitative differences occur in this enzyme between groups of cells of different sizes. The appearance of two new peroxidase isozymes is correlated with cellular differentiation as the cell mass enlarges from 0.5 to 4.0 mm in diameter.