Three-to-one segregation from reciprocal translocation quadrivalents inNeurosporaand its bearing on the interpretation of spore-abortion patterns in unordered asci

Abstract

In Neurospora, viable ascospores become black (B) when mature, whereas ascospores that are deficient for a chromosome segment are inviable and usually fail to blacken. The presence of a chromosome rearrangement can be recognized and the type of rearrangement can usually be inferred by visual inspection of asci. When a cross is heterozygous for a reciprocal translocation, asci with eight black ascospores (8B:0W) and asci with eight abortive unpigmented ("white" (W)) ascospores (0B:8W) are theoretically produced in equal numbers if homologous centromeres are equally likely to segregate from the quadrivalent in alternate or adjacent modes. In addition, 4B:4W asci are produced with a frequency characteristic of each reciprocal translocation. Information on ascospore-abortion patterns in Neurospora crassa has come predominantly from unordered ascospore octads ejected from the perithecium. Unordered asci of the 4B:4W type were initially presumed to originate by interstitial crossing over in a centromere-breakpoint interval and their frequency was used as a predictor of centromere locations. However, 4B:4W asci can result not only from interstitial crossing over but also from nondisjunction of centromeres at the first meiotic division, which leads to 3:1 segregation. Ordered linear 4B:4W asci retain the sequence information necessary for distinguishing one mode of origin from the other but unordered asci do not. Crossing over results in one abortive duplication–deficiency ascospore pair in each opposite half of a linear ascus, while 3:1 segregation places both abortive ascospore pairs together, either in the distal half or the basal half of the ascus. In the present study, perithecia were opened and intact linear asci were examined in crosses heterozygous for a varied sample of translocations. Three-to-one segregation rather than interstitial crossing over is apparently the main cause of 4B:4W asci when breakpoints are near centromeres, whereas crossing over is responsible for most or all 4B:4W asci when breakpoints are far-distal. Three-to-one segregation does not impair the usefulness of ejected unordered asci for detecting chromosome rearrangements. Ejected octads are superior to ordered linear asci for distinguishing one type of rearrangement from another, because ascus ejection from the perithecium does not occur until viable ascospores are fully pigmented, enabling true 0B:8W asci to be distinguished from those with eight immature ascospores.Key words: ascospore abortion, ascus analysis, Neurospora, nondisjunction, reciprocal translocation, three-to-one segregation.