A genetic map of common bean to localize specific resistance genes against anthracnose

Abstract

A bean genetic map was developed to locate resistance genes against anthracnose and genes involved in plant defense mechanisms. One hundred and fifty-seven markers (51 restriction fragment length polymorphism, 100 random amplified polymorphic DNA, 2 sequence characterized amplified regions, and 4 morphological markers) were used to construct a genetic map covering 567.5 cM of the bean genome. Morphological markers consisted in two resistance genes towards anthracnose (Are and RVI), a dominant gene for nuclear male sterility (Ms8) and a pod-shape character (SGou). This map was established by using a backcross population (BC1) of 128 individuals, derived from a cross between two European bean genotypes: Ms8EO2 and Corel. Nine percent of the markers showed segregation distortions and mapped to three regions. Clusters of 2–10 markers were observed in every linkage group. The possible origin of these clusters is discussed. Nineteen markers shared with a previously published bean linkage map allowed us to establish a preliminary correspondence between the two maps. Finally, seven genes involved in plant defense mechanisms were located on this map.Key words: Phaseolus vulgaris L., random amplified polymorphic DNA, restriction fragment length polymorphism, Colletotrichum lindemuthianum, male sterility.