Genetics of Glossina morsitans morsitans (Diptera: Glossinidae). XII. Comparison of field-collected and laboratory-reared flies

Abstract

Adult Glossina morsitans morsitans Westwood emerging from puparia collected at Rekometjie, Zimbabwe, were compared at 13 loci with G. m. morsitans from a closed colony at the Tsetse Research Labortory (TRL), University of Bristol, by polyacrylamide gel electrophoresis. The populations were not significantly different with respect to mean heterozygosity per locus, average effective number of alleles per locus, number of polymorphic loci, or allele frequencies at 12 loci. (The exception was Alkph.) Rare alleles at To, Est-t, and Alkph were found only in Rekometjie flies while rare alleles at G6pd and Est-2 were found only in TRL flies. No significant level of sterility was found in F1 flies produced in reciprocal crosses of Rekometjie flies with G. m. morsitans from the University of Alberta colony.Key words: Glossina, tsetse fly, isozymes, heterozygosity.