THREE MECHANISMS OF 2n POLLEN FORMATION IN DIPLOID POTATOES

Abstract

Three mechanisms of 2n pollen formation observed in diploid hybrids between Solatium tuberosum Group Phureja (2x) and haploids of S. tuberosum Group Tuberosum (4x) are termed parallel spindles (ps), premature cytokinesis 1 (pc 1) and premature cytokinesis 2 (pc 2). A II spindles are parallel to each other in many cells of clones producing 2n pollen by ps. A single cleavage furrow follows and forms a dyad which generates two 2n microspores. The other two mechanisms, pc 1 and pc 2, produce 2n pollen by omission of the second meiotic division. Premature cytokinesis 1 (pc 1) involves asynchronized movement of bivalents to and from the M I plate and falling apart of chromatids at T I. A cleavage furrow follows the first meiotic division, no second division occurs and products of meiosis are dyads. Premature cytokinesis 2 (pc 2) exhibits no abnormalities at the first meiotic division. However, cytokinesis occurs prematurely at P II, no second division occurs, and only dyads are formed. Genetic data suggested that these three mechanisms may be controlled by single loci, and are inherited independently as simple recessives.