Author:
Robinson H. M. Czajkowska,Rathbun J. C.
Abstract
A method for the determination of blood galactose in 0.1 ml. samples was developed in which the glucose is removed by treatment with yeast. Modified dilutions of Somogyi and Nelson reagents are used and the non-fermentable sugar is determined colorimetrically at 680 millimicrons. A comparison of three protein precipitants led to the choice of tungstic acid. The range covered by the method is 6–50 mg. %, corresponding to 2–16 micrograms of galactose per sample.
Publisher
Canadian Science Publishing
Cited by
3 articles.
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