Linkage relationships of allozyme-encoding loci in shiitake, Lentinula edodes

Abstract

Single spore derived isolates from two parental and seven hybrid lines of Lentinula edodes were analyzed for single locus and joint segregation of 21 allozyme-encoding loci. The two alleles at the individual loci departed significantly from a 1:1 Mendelian ratio in 11% of the tests. With the exception of the highly significant χ2 value for Aat-1 in line WC131 (χ2 = 41.78), aberrant ratios were marginally significant and probably were due to chance alone. Eight linkages were identified: Ada ~ Gda, recombination frequency (r) = 0.15; Ada ~ Est, r = 0.12; Est ~ Gpi, r = 0.29; Ada ~ Gpi, r = 0.35; Gdh ~ Sod-1, r = 0.18; Mpi ~ PepG1-2, r = 0.13; Mpi ~ Sod-2, r = 0.04; and Sod-2 ~ PepG1-2, r = 0.14. Twelve loci were not shown to be linked to any other loci (Aat-1, Ak, Cat, Dia, Gk, β-Glu-1, β -Glu-2, Mdh, PepLgg-2, Pgd, Pgm, and Np). The first linkage map of 10 allozyme-encoding loci for the L. edodes genome is presented.Key words: shiitake, Lentinula edodes, allozymes, linkage map, edible fungi.