Low growth temperature inhibition of photosynthesis in cotyledons of jack pine seedlings (Pinus banksiana) is due to impaired chloroplast development

Abstract

Cotyledons of jack pine seedlings (Pinus banksiana Lamb.) grown from seeds were expanded at low temperature (5°C), and total Chl content per unit area of cotyledons in these seedlings was only 57% of that observed for cotyledons on 20°C-grown controls. Chl a/b ratio of 5°C-grown jack pine was about 20% lower (2.3 ± 0.1) than 20°C controls (2.8 ± 0.3). Separation of Chl-protein complexes and SDS-PAGE indicated a significant reduction in the major Chl a containing complex of PSI (CP1) and PSII (CPa) relative to LHCII1 in 5°C compared to 20°C-grown seedlings. In addition, LHCII1/LHCII3 ratio increased from 3.8 in control (20°C) to 5.5 in 5°C-grown cotyledons. Ultrastructurally, 5°C-grown cotyledons had chloroplasts with swollen thylakoids as well as etiochloroplasts with distinct prolamellar bodies. Based on CO2-saturated O2 evolution and in vivo Chl a fluorescence, cotyledons of 5°C jack pine exhibited an apparent photosynthetic efficiency that was 40% lower than 20°C controls. Seedlings grown at 5°C were photoinhibited more rapidly at 5°C and 1200 µmol·m–2·s–1 than controls grown at 20°C, although the final extent of photoinhibition was similar. Exposure to high light at 5°C stimulated the xanthophyll cycle in cotyledons of both controls and 5°C-grown seedlings. In contrast to winter cereals, we conclude that growth of jack pine at 5°C impairs normal chloroplast biogenesis, which leads to an inhibition of photosynthetic efficiency.Key words: chloroplast, growth, temperature, photosynthesis, photoinhibition, Pinus banksiana Lamb., ultrastructure.