ACTIVITY AND RELATIVE MOBILITY OF PEROXIDASE AND ESTERASE ISOZYMES OF FLAX (LINUM USITATISSIMUM) GENOTROPHS. II. F1 HYBRIDS AND NUCLEAR DNA REVERSION TYPES

Abstract

The effects of growth of one genotype of flax in soil supplemented by either nitrogen, phosphorous and potassium (NPK) or nitrogen and potassium (NK) on its progeny produced by several generations of complete selfing were studied. The two types of progeny produced, genotroph L, induced by NPK and genotroph S induced by NK, and their F1 reciprocal hybrids were examined. L and S differed in plant weight and in the activities and relative mobilities of their corresponding anionic peroxidase and esterase isozymes. No reciprocal differences were detected and the mean F1 values for all characteristics were intermediate between the parents, with the exception of the peroxidase isozyme relative mobilities which displayed dominance of parent L. The genotrophs were known to exhibit a 16% difference in nuclear DNA content which could be reverted to 0%, without altering the genotroph fresh weight difference, by growing the genotrophs in lower than normal temperatures. Examination of the peroxidase and esterase isozymes of nDNA reverted genotrophs showed that, with one possible exception, there was no accompanying reversion of the activities and relative mobilities of the peroxidase or esterase isozymes.