Author:
Boucher Roger,Gauthier Marie,Jolicoeur Paul,Labrie Fernand
Abstract
Actinomycin D, at doses (25 and 50 μg/ml) that block RNA synthesis to less than 3% of the control rate, inhibits the incorporation of [3H]leucine into adenohypophyseal proteins and the release of newly synthesized proteins by 50 and 60%, respectively, of the control rates. Despite this lowering of basal levels of total protein synthesis and release in presence of the antibiotic, the percentage of stimulation of both protein synthesis and release by 5 mM N6-2′-O-dibutyryl adenosine 3′5′-monophosphate (dbcAMP) is not depressed by actinomycin D. When rat hemipituitaries are incubated with [3H]uridine, dbcAMP does not stimulate the labeling of total cytoplasmic RNA or the preferential labeling of any cytoplasmic RNA species resolved on sucrose gradient. There is no stimulatory effect of dbcAMP on total labeling or preferential incorporation into nuclear RNA species extracted at 24 °C or at 65 °C. Labeling of the nucleotide pools was unchanged up to 1 h of incubation but was increased (40–70%) during the last [Formula: see text] of incubation. These data suggest that the short-term stimulatory effects of dbcAMP on total adenohypophyseal protein synthesis and release are exerted at the transiational level.
Publisher
Canadian Science Publishing
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献