Chemically defined media for Pasteurella multocida and Pasteurella ureae, and a comparison of their thiamine requirements with those of Pasteurella haemolytica

Abstract

Chemically defined media supported heavy cell yields of Pasteurella multocida and moderate yields of P. ureae (P. haemolytica var ureae). The medium for P. multocida consisted of 17 amino acids, plus inorganic salts, citrate, nicotinamide, pantothenate, thiamine or thiamine monophosphate, adenine, guanine, uracil, and energy source; the medium for P. ureae was the same except that it contained an additional amino acid. Six strains of P. multocida, five of P. ureae, and two of P. haemolytica grew well with normal amounts of thiamine; however, 9 of 11 strains of P. haemolytica could satisfy their thiamine requirement with free thiamine only if large amounts of the vitamin were provided. All strains used thiamine monophosphate or thiamine pyrophosphate efficiently. Pasteurella multocida grew well with a combination of the thiamine moieties, 4-methyl-5(β-hydroxyethyl)thiazole, and 2-methyl-4-amino-5-hydroxymethyl pyrimidine, or with their phosphate esters; P. haemolytica grew only if the phosphates were provided.