Chromosomal location of genes controlling lactate dehydrogenase in rye, wheat, and barley

Abstract

The chromosomal location of lactate dehydrogenease (LDH) genes in rye, barley, and wheat was investigated using the disomic standard addition sets of 'Chinese Spring' – 'Imperial' (wheat–rye) and 'Chinese Spring' – 'Betzes (wheat–barley) and the monosomic rye–wheat additions, respectively. Gel electrophoresis was performed on 5% polyacrylamide gels. Diagnostic bands for LDH isozymes were present only in zone 2. Genes controlling LDH isozymes in rye were found to be located on rye chromosome 4R (Ldh-R1and Ldh-R2) and in barley on chromosome 4Hv (Ldh-H1 and Ldh-H2). Because of the incomplete rye–wheat addition set, only one gene for LDH isozymes of wheat could be located (wheat chromosome 4B; Ldh-B1). In the three cereal species investigated, LDH genes are located generally on homoeologous group 4 chromosomes.Key words: gene location, lactate dehydrogenase, Secale cereale, Triticum aestivum, Hordeum vulgare.