Detoxification of secondary products of lipid peroxidation in the cytosol of a mouse fibroblast cell line

Abstract

A cytosolic fraction prepared from a mouse fibroblast cell line reduced secondary products of lipid peroxidation, including alkenals, alkanals, alk-2-enals, and alka-2,4-dienals, using NADH or NADPH. An alternative route for detoxification of alk-2-enals and alka-2,4-dienals in the cell line cytosol was via glutathione conjugation. The major glutathione transferase in the cell line cytosol was partially characterized by glutathione-agarose chromatography and chromatofocusing. High glutathione peroxidase activity suggested that the enzyme was an alpha family glutathione transferase. The major glutathione transferase also efficiently catalyzed the conjugation of alk-2-enals and alka-2,4-dienals with glutathione.Key words: lipid peroxidation, glutathione transferase, detoxification.