Characterization and control of endophytic bacterial contaminants in in vitro cultures of Piper spp., Taxus baccata subsp. wallichiana, and Withania somnifera

Abstract

Bacterial contamination is a serious problem that causes severe loss of in vitro grown cultures of a number of plants. This problem becomes even more acute if the bacterial contamination is of endophytic origin. In such cases, identification and characterization of the contaminants is essential for achieving specific control of the contaminants through selective use of antibiotic agents, especially if the routinely used contamination control methods practiced elsewhere in tissue culture studies are ineffective. Such is the case with the bacterial contamination observed in the present study. The five endophytic bacteria associated with Piper nigrum and Piper colubrinum, four endophytic bacteria associated with Taxus baccata subsp. wallichiana, two endophytic bacteria associated with Withania somnifera, and two bacteria common to all these plant species were isolated and characterized based on morphological and biochemical tests. Their taxonomic positions based on similarity indices were determined. A control strategy against these bacteria has been developed based on bacteriostatic or bactericidal actions of 12 antibiotics at three different concentrations by solid and liquid antibiogramme assays.Key words: antibiotics, endophytic bacterial contamination, identification, Piper nigrum, Piper colubrinum, Taxus baccata subsp. wallichiana, Withania somnifera.