2-Deoxyglucose Transportation Via Passive Diffusion and its Oxidation, Not Phosphorylation to 2-Deoxygluconic Acid by Pseudomonas aeruginosa

Abstract

The transport and catabolism of 2-deoxyglucose by Pseudomonas aeruginosa were studied. 2-Deoxyglucose was taken up and oxidized by glucose-grown cells at a rate approaching that of the uptake and oxidation of glucose. However, 2-deoxyglucose entered these cells via passive diffusion while glucose entered via an inducible active transport system. 2-Deoxyglucose was oxidized stoichiometrically, presumably by glucose dehydrogenase, to 2-deoxygluconic acid which, in turn, diffused out of the cells. No phosphorylated intermediates were detected. Glucose dehydrogenase was induced by cultivating the cells on glucose. There was suggestive evidence that the membrane-bound glucose dehydrogenase provided energy for the transport of glucose.