Strain construction for enhanced production of spinosad via intergeneric protoplast fusion-Reference-Cited by-同舟云学术

Strain construction for enhanced production of spinosad via intergeneric protoplast fusion

Published:2009-09 Issue:9 Volume:55 Page:1070-1075
ISSN:0008-4166
Container-title:Canadian Journal of Microbiology
language:en
Short-container-title:Can. J. Microbiol.

Author:

Wang Chao¹²,Zhang XiaoLin¹²,Chen Zhi¹²,Wen Ying¹²,Song Yuan¹²

Affiliation:

1. Department of Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100193, China.

2. Academy of State Administration of Grain, Beijing 100037, China.

Abstract

Spinosad is a new class of insecticides produced by Saccharopolyspora spinosa . The aim of this study was to construct a starch-utilizing strain that overproduced spinosad by intergeneric fusion between S. spinosa and Streptomyces avermitilis . Protoplast fusion is an important technique for engineering microbial strains, especially for microorganisms with few available molecular genetic tools. Protoplast fusion was conducted with UV-irradiated protoplasts of S. spinosa and S. avermitilis. Among 76 recombinants screened by ESI-MS and HPLC, a starch-utilizing strain F17, identified as S. spinosa, was obtained. The yield of spinosad in F17 was increased by 447.22%, compared with the yield of the wild-type strain. This is the first report of intergeneric protoplast fusion between S. spinosa and S. avermilitis, which shows great potential for industrial applications.

Publisher

Canadian Science Publishing

Subject

Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology

Link

http://www.nrcresearchpress.com/doi/pdf/10.1139/W09-064

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