Natural and experimental nematode infections in red deer (Cervus elaphus elaphus) and the potential for antemortem serodiagnosis of the tissue worm Elaphostrongylus cervi

Abstract

Red deer (Cervus elaphus elaphus) were exposed to a variety of nematodes, either naturally on pasture (n = 12) or experimentally under controlled conditions (n = 30). Experimental exposures included a combination of one or more of Elaphostrongylus cervi, Dictyocaulus sp., and Muellerius capillaris. The prepatent period of E. cervi infections was 92–133 days post exposure (dpe) in 12 deer each given 20–42 infective larvae (L3) and maintained under controlled conditions. Adult E. cervi were recovered from all 12 animals at necropsy. The prepatent period of Dictyocaulus sp. was 23–37 dpe in 10 deer each given 100 L3 and maintained under controlled conditions. Adult Dictyocaulus sp. were recovered from seven animals at necropsy. No animal exposed to 42–54 M. capillaris L3 developed patent infections, nor were adult worms recovered at necropsy. There was no evidence of neurologic signs in any deer at any time during the experiment. An enzyme-linked immunosorbent assay (ELISA) using somatic protein extracts of adult E. cervi or those from the closely related nematode Parelaphostrongylus tenuis was evaluated. Although the ELISA was sensitive, it lacked specificity with heterologous infections. However, the close phylogenetic relationship of E. cervi to P. tenuis, and our ELISA results, suggest that molecules from P. tenuis may represent a viable alternative source for use in the future development of a reliable antemortem serodiagnostic assay for E. cervi.