The purification and properties of valine tRNAs of Drosophila melanogaster

Abstract

The valine transfer ribonucleic acids of Drosophila melanogaster have been purified by column chromatography on BD-cellulose, Sepharose 6B, and RPC-5. Three major species were analyzed for base composition and coding properties. [Formula: see text] binds strongly to ribosomes in the presence of GUA and to a lesser extent with GUU and GUG. [Formula: see text] binds strongly in the presence of GUG and very poorly if at all with the other three triplets whereas [Formula: see text], which contains inosine, binds strongly in the presence of GUU, GUC, and GUA and weakly with GUG.