Assembly of Complex III into Newly Developing Mitochondrial Membranes

Abstract

Yeast cells grown anaerobically in 0.02% linoleic acid were transferred to air in the presence of 0.02% elaidic acid. At varying times Arrhenius plots were made of QH2-cytochrome c reductase activities in isolated mitochondria. A transition temperature of 8.2 °C at 0.5 h was characteristic of linoleate; at 3 h the transition temperature was increased to 24 °C characteristic of elaidate. At early times the enzyme was associated with anaerobic promitochondrial membranes; at later stages the newly synthesized enzyme was associated with newly developed elaidate membranes.