Hemicellulose as a potential substrate for production of poly(β-hydroxyalkanoates)

Abstract

Pseudomonas cepacia was evaluated for its ability to utilize xylose, a major hemicellulosic sugar of hardwoods, for the production of the biodegradable, thermoplastic poly(β-hydroxybutyrate) (PHB). This culture produced 2.6 g∙L−1 of biomass containing 60% (w/w) PHB when grown in shake flasks on an ammonium-limited, mineral salts medium containing 10 g∙L−1 of xylose. Batch fermentation data showed that growth and PHB production kinetics on xylose were similar to previously published results for the same microorganism on fructose. On xylose, the maximum specific growth rate, the maximum specific PHB production rate (based on total biomass minus PHB biomass), the overall yield of biomass produced from substrate consumed, the yield of PHB produced from substrate consumed (YPHB/S), and the percentage of PHB were 0.22 h−1, 0.072 g∙g−1∙h−1, 0.29 g∙g−1, 0.11 g∙g−1 and 45% (w/w), respectively. A high maintenance energy (0.119 g of xylose∙g of biomass−1∙h−1) is probably responsible for the low overall yield. However, the product yield, YPHB/S, was still the highest reported for any microorganism grown on pentosic sugars. Using the YPHB/S of 0.11 g∙g−1, it was estimated that the substrate cost (in terms of hydrolyzed hemicellulose) for PHB production would be similar to that of cane molasses and half that of bulk glucose.Key words: poly(β-hydroxybutyrate), PHB, hemicellulose, xylose, Pseudomonas cepacia.