"PROTOPLASTS" FROM PYTHIUM SP. PRL 2142

Abstract

Enzyme preparations from two Streptomyces spp. released spherical protoplast-like bodies from the mycelium of Pythium sp. PRL 2142. The enzyme complexes were produced by growing the Streptomyces spp. in the presence of autoclaved Pythium mycelium, and concentrated from the culture mixture by precipitation with acetone (2:1, v/v). The protoplasts were released, from both intercalary and terminal locations on the hyphae, during incubation with the lytic enzyme complex for 24 hours using 0.8 M MgSO4 as an osmotic stabilizer.