Abstract
The purpose of this investigation was to determine the total lipide, neutral fat, total fatty acid, total cholesterol, ester cholesterol, free cholesterol, and phospholipide content, estimated by oxidative micromethods, of human plasma, pooled, irradiated, and dried, as collected by the Canadian Red Cross Blood Transfusion Service and processed at the Connaught Medical Research Laboratories. Determinations were made before and after storage as dried plasma for six months in the dark at temperatures of −40° C., 4° C., 20° C., and 40° C. All lipides present in this dry powdered plasma were found to be completely taken up in the distilled water used for its reconstitution to liquid plasma. The reconstituted liquid plasma contained approximately one-half the concentration of lipides present in fresh normal human plasma, the difference being due entirely to dilution during preparation and processing, except in the instances of both free and esterified cholesterol, of which approximately one-fifth the amount present in fresh normal human plasma was found to be missing in the final product. No statistically significant loss of lipides in dried plasma occurred after storage at −40° C., 4° C., or 20° C.; at 40° C. there was a statistically significant loss of approximately one-fifth of practically all lipides. Ultraviolet irradiation during processing did not affect the lipide composition of dried plasma determined before and after storage at 4° C.
Publisher
Canadian Science Publishing