HEXOSE PHOSPHATE METABOLISM BY ACETOBACTER MELANOGENUM

Author:

Katznelson H.

Abstract

Cell-free preparations of Acetobacter melanogenum readily oxidized glucose-6-phosphate, glucose-1-phosphate, fructose-6-phosphate, 6-phosphogluconate, and ribose-5-phosphate; fructose-1,6-diphosphate was utilized very slowly. The presence of an active triphosphopyridine nucleotide (TPN)-linked glucose-6-phosphate dehydrogenase and of phosphohexose isomerase was demonstrated; phosphoglucomutase was also present in these extracts. 6-Phosphogluconate dehydrogenase activity (TPN-linked) was low in extracts of glucose-grown cells, but was high in sonates of gluconate-grown cells. An active oxidizing system for reduced diphosphopyridine nucleotide was present but transhydrogenase was not detected. Pyruvate was produced extensively from 6-phosphogluconate but only slowly from ribose-5-phosphate; fluoride inhibited pyruvate formation from both substances. Ribose-5-phosphate was degraded readily via the transketolase-transaldolase series of reactions. Glyceraldehyde-3-phosphate dehydrogenase, hexokinase, gluconokinase, 2-ketogluconokinase, and aldolase were detected but not phosphohexokinase. The results suggest that glucose is oxidized either directly or after phosphorylation but is not metabolized glycolytically. The oxidation of hexose phosphate appears to occur predominantly as a result of the splitting of 6-phosphogluconate to pyruvate and glyceraldehyde-3-phosphate.

Publisher

Canadian Science Publishing

Subject

Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology

Cited by 16 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3