Some characteristics of streptococci competent for uptake of deoxyribonucleic acid

Abstract

The degree of competence for uptake of deoxyribonucleic acid (DNA uptake) of the Challis streptococcus was found to be strictly related to the concentration of cell-bound competence-provoking factor (CPF). Antibodies against competent cells and antibodies against crude concentrates of CPF inhibited DNA uptake by competent cells. Both types of antibodies interacted also with soluble CPF and reduced its capacity to induce competence. Two characteristic features of competent streptococci were revealed: high precipitability at low pH's and a reduced capacity to absorb methylene blue.