Production of aerial mycelium and teliospores of Puccinia horiana in chrysanthemum callus cultures

Abstract

Chrysanthemum callus tissues were grown from leaf pieces infected with Puccinia horiana. Feltlike aerial mycelia were frequently produced on the callus tissue. Callus tissue with aerial mycelium was maintained in vitro for more than 1 year by transplants at 4-month intervals. Teliospores were produced on the callus surface, in enlarged intercellular spaces of callus tissue, and in callus cells. Teliospores were longer than normal and had one to three cells instead of two cells. Aerial mycelium on callus tissue infected excised host leaves.