Author:
Pulina Maria O,Zakharova Elena T,Sokolov Alexei V,Shavlovski Mikhail M,Bass Mikhail G,Solovyov Kirill V,Kokryakov Vladimir N,Vasilyev Vadim B
Abstract
We have previously shown that iron-containing human lactoferrin (LF) purified from breast milk is able to form both in vitro and in vivo a complex with ceruloplasmin (CP), the copper-containing protein of human plasma. Here we present evidence that the CPLF complex is dissociated by high concentrations of NaCl, CaCl2, or EDTA, or by decreasing the pH to 4.7. In addition, DNA, bacterial lipopolysaccharide, and heparin can displace CP from its complex with LF. Antibodies to either of the two proteins also cause dissociation of the complex.Key words: lactoferrin, ceruloplasmin, ferroxidase.
Publisher
Canadian Science Publishing
Subject
Cell Biology,Molecular Biology,Biochemistry
Cited by
28 articles.
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