Author:
Macdonald J. B.,Madlener E. M.
Abstract
Three approaches were used in attempting to improve the method of isolating Spirillum sputigenum from the human oral cavity:(1) attempts to induce spreading surface growth;(2) selective inhibition of interfering organisms;(3) adjustment of oxidation–reduction potentials.Five of eight strains of Spirillum sputigenum grew as spreading surface films on a blood agar medium in which the base was a veal heart infusion. Increase in the amount of spread could not be induced by varying the agar content of the medium. Sodium lauryl sulphate (0.01%) was found to have a marked inhibitory effect on the human oral flora but did not inhibit Spirillum sputigenum. A method of recording potentials of the surface layer of solidified media using a gold foil electrode is described. Changes in potential of the surface layer of a number of media during reduction in a Brewer jar are recorded. Media in which the potential was rapidly reduced supported growth of Spirillum sputigenum. Of several reducing substances added to media, sheep's serum was the most effective in accelerating a drop in potential. Using a medium compounded of veal heart infusion, sodium lauryl sulphate, and sheep's serum, Spirillum sputigenum was recovered in pure culture from 16 out of 21 samples of gingival scrapings.
Publisher
Canadian Science Publishing
Subject
Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology
Cited by
9 articles.
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