Location of α-amylase/subtilisin inhibitor during kernel development and germination

Abstract

The location of an endogenous α-amylase/subtilisin inhibitor in developing and germinating barley (Hordeum vulgare, cv. Bonanza) was determined using immunohistochemical techniques. The inhibitor was found within protein bodies of cells containing starch granules in the starchy endosperm and embryo of developing caryopses. It could be detected as early as 2 weeks postanthesis in both organs. Upon germination, inhibitor was released from protein bodies, resulting in increased detection of the protein in regions of the starchy endosperm in which storage mobilization was occurring. Antibodies to α-amylase revealed large quantities of this protein in the same regions. Key words: α-amylase, α-amylase/subtilisin inhibitor, barley, germination, kernel development, starch.