Antigenic studies on coronavirus. I. Identification of the structural antigens of human coronavirus, strain 229E
-
Published:1981-03-01
Issue:3
Volume:27
Page:334-342
-
ISSN:0008-4166
-
Container-title:Canadian Journal of Microbiology
-
language:en
-
Short-container-title:Can. J. Microbiol.
Author:
Yaseen Shehab A.,Johnson-Lussenburg C. Margaret
Abstract
Antigenic analysis of human coronavirus, strain 229E (HCV/229E), using a microimmunodiffusion technique, has resulted in the detection of six virion antigens. Comparison of the effect of several different virus-disrupting agents has shown that sodium deoxycholate or Triton X-100 were the best for HCV/229E disruption. Of the six coronavirion antigens, three were identified as virus specific and the remainder as host antigens, which were present as either integrated or nonspecifically adsorbed host components. One of the virus-specific antigens was identified as the internal nucleoprotein (229E/RNP). On the basis of immunodiffusion reactions with isolated 229E/RNP it was concluded that human convalescent sera reacted specifically with the 229E/RNP antigen.
Publisher
Canadian Science Publishing
Subject
Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
1. The Biology of Coronaviruses;Journal of General Virology;1983-04-01
2. The Biology and Pathogenesis of Coronaviruses;Current Topics in Microbiology and Immunology;1982