Optimization of 7α-hydroxysteroid dehydrogenase production by Escherichia coli 080
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Published:1990-10-01
Issue:10
Volume:36
Page:725-727
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ISSN:0008-4166
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Container-title:Canadian Journal of Microbiology
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language:en
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Short-container-title:Can. J. Microbiol.
Author:
Prabha V.,Gupta Meenakshi,Gupta K. G.
Abstract
7α-Hydroxysteroid dehydrogenase (EC 1.1.1.159) production by Escherichia coli strain 080 was highest when the organism was grown in brain heart infusion broth at pH 6.5 for 72–96 h with shaking at 37 °C. The oxygen consumption rate had a strong effect on the production of this constitutive enzyme. Glucose and lactose at 0.2–0.4%, detergents, and ethylenediaminetetraacetic acid were found to increase the enzyme production. Key words: 7α-hydroxysteroid dehydrogenase, Escherichia coli 080, optimal conditions for 7α-hydroxysteroid dehydrogenase production.
Publisher
Canadian Science Publishing
Subject
Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology
Cited by
1 articles.
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