Rhizomorph exudate of Armillaria mellea

Abstract

Droplets of exudate from rhizomorphs of Armillaria mellea (Vahl ex Fr.) Kummer, grown on carrot agar, grew larger in size and darkened with age. The exudate was found to contain free amino acids, protein, and inorganic elements, but no oxalic acid was detected. β-Glucosidase, peroxidase, polyphenoloxidase, and acid and alkaline protease activities were also detected. Gel electrophoresis was used to analyze the dark brown exudate. A single dark brown band was obtained using the disc gel procedure, whereas many brown bands were formed using the isoelectric focusing technique.