Chromosomal localization of the white gene of Lucilia cuprina (Diptera; Calliphoridae) by in situ hybridization

Abstract

The white gene of Lucilia cuprina was mapped to trichogen polytene chromosomes using in situ hybridization. A tritium-labelled riboprobe made from the first gene cloned from this species was used with techniques modified from standard methods used for Drosophila melanogaster. Cytological data limiting the location of the white gene to a small portion of 3L and complementing the in situ results are also presented. Key words: Lucilia cuprina, white gene, in situ hybridization.