Stimulation of Waitea circinata by root exudates of Pinus cembroides

Author:

Agnihotri V. P.,Vaartaja O.

Abstract

Effects of root exudates from Pinus cembroides to Waitea circinata were studied by using paper chromatography. Each of 14 seedlings liberated an average of 175 μg of ninhydrin-positive substance and 133 μg of sugars in 35 days. From these, 14 amino acids, 4 sugars, and 3 organic acids were identified. The root exudate induced greatly increased mycelial growth over a wide temperature range, but sclerotia were produced only after 20 days between 15 and 20 °C. Each amino acid and carbohydrate, except threonine, valine, glycine, glutamic acid, lysine, γ-aminobutyric acid, and citric acid, stimulated vegetative growth. Neither basidia nor sclerotia developed on any nutrient medium. The sclerotia did not require exogenous nutrition for germination and initial growth. Germination percentage remained between 95 and 100 with each exudate component. All carbohydrate components, either singly or in combination, stimulated extension growth from sclerotia. Of 14 amino acids, only aspartic acid, glutamic acid, and leucine supported faster extension growth than the control (water agar). Temperature and pH influenced germination and growth, the optima being at 25 to 30 °C and pH 5 to 6.

Publisher

Canadian Science Publishing

Subject

Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3