Ultrastructure of uterine trophonemata, accommodation for uterolactation, and gas exchange in the southern stingray, Dasyatis americana

Abstract

The southern stingray, Dasyatis americana, displays aplacental viviparity, embryos being retained in the maternal uterus throughout gestation and initially nourished by the yolk sac contents. During gestation the uterus develops vascularized appendages, trophonemata, that secrete viscous nutrient histotroph that is subsequently ingested by the embryo as it grows to term. There is a 3750% increase in wet mass from the egg to the term fetus. Trophonemata are 1.5 cm long, narrower at the base, and spatulate at the tip. Surface epithelial cells form a pattern of surface cables, each with a small blood vessel at its core. In females containing fertilized eggs, the epithelium is simple and cuboidal. In contrast, in uteri containing late-term fetuses, the epithelium is squamous. Epithelial cells, with periodic acid – Schiff positive cytoplasmic vesicles, form invaginated crypts. Epithelial cells produce proteinaceous, mucous, and lipid secretions, thus we have coined the term uterolactation to describe this phenomenon.