Conditions affecting the amphotericin B mediated inhibition of Candida albicans attachment to cell cultures

Abstract

Sublethal amounts of amphotericin B inhibited the attachment of Candida albicans to cultured mammalian cells. The extent of inhibition was influenced by the concentration of serum and the growth phase of the yeasts used to inoculate the cell cultures. Yeasts which were in their exponential phase of growth or had formed germ tubes were the most sensitive to amphotericin B. Equivalent amounts of amphotericin B inhibited yeast – mammalian cell interactions to different degrees depending upon the culture's tissue origin.Key words: Candida, amphotericin, polyenes, attachment, yeasts.