Inoculation of western white pine tissue cultures with basidiospores of Cronartium ribicola

Abstract

Rust infections were established in healthy, undifferentiated tissue cultures of Pinus monticola Dougl. by direct inoculation with germinating basidiospores of Cronartium ribicola J. C. Fisch. ex Rabenh. Infections were verified by confirming the presence of typical rust haustoria and extensive intracellular ramification by the mononucleate rust mycelium.