A study of the molecular–cellular interface in visual photoreceptors by light scattering photometry

Author:

Borys T.,Deshpande S.,Jones R.,Abrahamson E. W.

Abstract

The correlation of molecular events with structural changes within the cell requires a non-destructive relaxation technique that can be adapted to measure such cellular changes in a time range of milliseconds to minutes. Light scattering relaxation techniques have proved useful for such studies as they can often be measured simultaneously or in parallel with absorption or fluorescence spectral changes characterizing molecular or macromolecular processes. Such techniques are proving useful in the study of photobiological processes such as visual photoreception where specific cytological changes produced photochemically can be effected by alternate controlled perturbations such as osmotic shrinking or swelling of cell organelles and (or) whole cells. This paper illustrates how light scattering relaxation spectrophotometry can be applied to the correlation of molecular and cellular events in visual photoreceptors.

Publisher

Canadian Science Publishing

Subject

Organic Chemistry,General Chemistry,Catalysis

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